Chap 27 Bacteria and Archaea

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A prokaryote such as this E. coli cell has a circular chromosome consisting of a single ring of DNA, located in a nucleoid region. Many cells also harbor smaller, non-genome rings of DNA called plasmids.

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Metabolic cooperation in a colonial prokaryote.
In the filamentous cyanobacterium Anabaena, cells known as heterocytes fix nitrogen, while the other cells carry out photosynthesis, and these cells exchange metabolic products.

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The polysaccharide or protein capsule surrounding the cell wall of this Streptococcus enables the bacterium to attach to cells that line the human respiratory tract, such as a tonsil cell. Other prokaryotes stick to the substrate by fimbriae.

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Cyanobacteria ("Blue-green algae") are photosynthetic and can form stromatolites. These two species of Oscillatoria form a filamentous colony. _Vid_Campbell7e/Cyanobacteria-V.swf
Some filamentous colonies such as Anabaena have cells specialized for nitrogen fixation.

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A comparison of the three domains of life. Among the prokaryotes, Aside from not having organelles such as the nucleus, and having circular DNA, Archaea exhibit many traits not shared with Bacteria.

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An endospore. Bacillus anthracis, the bacterium that causes anthrax, produces persistent endospores. An endospore's thick, protective coat helps it survive in the soil for years.
_Vid_Discover2e/ch09a05_Anthrax.swf

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Some prokaryotes attach to surfaces or to other prokaryotes via numerous protein appendages called fimbriae.

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Prokaryotic flagellum. Unlike an eukaryote flagellum, the motor of the prokaryotic flagellum is a basal apparatus embedded in the plasma membrane. A curved hook turns in the basal apparatus, moving the attached filament, which is composed of the protein flagellin. This allows the cell to responding to a stimulus in a directional movement called taxis.

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Gram-Positive Bacteria
Soil-dwelling Streptomyces are a source of many antibiotics, including streptomycin used to treat tuberculosis, which is caused by another gram-positive bacteriun, Mycobacterium tuberculosis Mycoplasmas are tiny and have no cell walls. One species causes "walking pneumonia". Hundreds of mycoplasmas are seen here covering a human fibroblast cell.

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Bacteria are stained with a violet dye and iodine, rinsed in alcohol, and then stained with a red dye.
Gram-positive bacteria have a cell wall with a large amount of peptidoglycan that traps the violet dye in the cytoplasm, masking the added red dye. Gram-negative bacteria have less peptidoglycan. The violet dye is easily rinsed from the cytoplasm, and the cell appears red after the red dye is added.

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Extreme halophiles.
Colorful “salt–lovingarchaea thrive in these ponds near San Francisco. Used for commercial salt production, the ponds contain water that is five to six times as salty as seawater.

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Lyme disease.
Ticks in the genus Ixodes spread the disease by transmitting the spirochete Borrelia burgdorferi. A large, ring–shaped rash may develop at the site of the tick's bite.

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Specialized membranes of prokaryotes.
Infoldings of the plasma membrane, reminiscent of the cristae of mitochondria, function in cellular respiration in some aerobic prokaryotes. Photosynthetic prokaryotes called cyanobacteria have thylakoid membranes, much like those in chloroplasts.

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Prokaryotes do not constitute a monophyletic clade. Archaea are more closely related to Eukarya than to Bacteria.

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Some bacteria have small rings of non-genome DNA called plasmids.

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Surrounding the plasma membrane, bacteria possess a cell wall composed of varying amounts of peptidoglycan, a polymer made of amino acids and sugars.

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Escherichia coli is a proteobacterium in the Gram-negative group.

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Rhizobium is another proteobacterium among the Gram-negative bacteria. They live inside root cells of legumes (plants of the pea/bean family), where the bacteria convert atmospheric nitrogen to organic compounds such as ammonia in a process called nitrogen fixation.

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Cocci (singular, coccus) are spherical prokaryotes. They occur singly, in pairs (diplococci), in chains (such as these streptococci), and in clusters like bunches of grapes (staphylococci). Bacilli (singular, bacillus) are rod-shaped prokaryotes. They are usually solitary, sometimes the rods are arranged in chains (streptobacilli). Spiral prokaryotes include spirilla, which range from comma-like shapes to long coils, and spirochetes (shown here), which are corkscrew-shaped.

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Extreme thermophiles. Orange and yellow colonies of “heat–lovingarchaea in the hot water of a Nevada geyser.