Whole-genome shotgun approach to sequencing.

In this approach, developed by Celera Genomics, random DNA fragments are sequenced and then ordered relative to each other.

  1. Cut the DNA from many copies of an entire chromosome into overlapping fragments short enough for sequencing.

  2. Clone the fragments in plasmid or phage vectors

  3. Sequence each fragment

  4. Order the sequences into one overall sequence with computer software.