The recombination frequencies between 3 gene pairs (b-cn 9%, cn-vg 9.5%, and b-vg 17%) best fit a linear order in which cn is positioned about halfway between the other 2 genes.

Recombination frequencies can be used to construct a linkage map of the chromosome, since the farther apart genes are, the more likely they are to be separated during crossing over, and the higher their frequency of recombination.